Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic bone repair

Abstract Cell therapy associated with guided bone regeneration (GBR) can be used to treat bone defects under challenging conditions such as osteoporosis. This study aimed to evaluate the effect of mesenchymal stem cells (MSCs) in combination with a poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on bone repair in osteoporotic rats. Osteoporosis was induced in female rats by bilateral removal of the ovaries (OVX) or sham surgery (SHAM), and the osteoporotic condition was characterized after 5 months by microtomographic and morphometric analyses. Calvarial defects were created in osteoporotic rats that immediately received the PVDF-TrFE/BT membrane. After 2 weeks, bone marrow-derived MSCs from healthy rats, characterized by the expression of surface markers using flow cytometry, or phosphate-buffered saline (PBS) (Control) were injected into the defects and bone formation was evaluated 4 weeks post-injection by microtomographic, morphometric, and histological analyses. A reduction in the amount of bone tissue in the femurs of OVX compared with SHAM rats confirmed the osteoporotic condition of the experimental model. More bone formation was observed when the defects were injected with MSCs compared to that with PBS. The modification that we are proposing in this study for the classical GBR approach where cells are locally injected after a membrane implantation may be a promising therapeutic strategy to increase bone formation under osteoporotic condition.

Marginal and internal fit of zirconia copings obtained using different digital scanning methods

Abstract The objective of this study was to evaluate the marginal and internal fit of zirconia copings obtained with different digital scanning methods. A human mandibular first molar was set in a typodont with its adjacent and antagonist teeth and prepared for an all-ceramic crown. Digital impressions were made using an intraoral scanner (3Shape). Polyvinyl siloxane impressions and Type IV gypsum models were also obtained and scanned with a benchtop laboratory scanner (3Shape D700). Ten zirconia copings were fabricated for each group using CAD-CAM technology. The marginal and internal fit of the zirconia copings was assessed by the silicone replica technique. Four sections of each replica were obtained, and each section was evaluated at four points: marginal gap (MG), axial wall (AW), axio-occlusal edge (AO) and centro-occlusal wall (CO), using an image analyzing software. The data were submitted to one-way ANOVA and Tukey's test (α = 0.05). They showed statistically significant differences for MG, AO and CO. Regarding MG, intraoral scanning showed lower gap values, whereas gypsum model scanning showed higher gap values. Regarding AO and CO, intraoral digital scanning showed lower gap values. Polyvinyl siloxane impression scanning and gypsum model scanning showed higher gap values and were statistically similar. It can be concluded that intraoral digital scanning provided a lower mean gap value, in comparison with conventional impressions and gypsum casts scanned with a standard benchtop laboratory scanner.

Influence of different fillers on the properties of an experimental vinyl polysiloxane

Abstract The aim of the study was to evaluate the effect of the incorporation of different fillers on an experimental vinyl polysiloxane (VPS) at two different concentrations, 20% and 40%. Different fillers were added to an experimental VPS. The study was developed in two stages: (i) incorporation of fillers in different concentrations: (a) 20 wt% fillers, and (b) 40 wt%. The fillers were added to experimental VPS and mixed with a speed mixer; (ii) characterization of experimental VPS; after the base paste and catalyst paste were mixed, the experimental VPS was used to make specimens specifically for each test, which were stored at 23°C for 24 hours. The tests were designed according to the specific standardization for the analysis of tensile strength, detail reproduction, Shore A hardness, and elastic recovery. For analysis of filler size pattern, scanning electron microscopy at 1500× magnification was used. The aerosil OX-50 40% (AE), and pure aluminum hydroxide 40% (PAH) groups presented the highest tensile strength and Shore A hardness values. However, those were the only groups that did not present continuous detail reproduction of an intersection of 20 μm line. The elastic recovery was not statistically significant. The undesirable characteristics of VPS (lowest Shore A hardness and tensile strength) were observed when it was added to the composition of acrylic polymer (AP) and fiberglass (FG) in both concentrations, 20% and 40%. In groups AE and PAH, agglomerates of nanofillers were shown in SEM micrography, while the other groups presented different shapes and fillers sizes.

Biocompatibility of human auricular chondrocytes cultured onto a chitosan/polyvynil alcohol/epichlorohydrin-based hydrogel for tissue engineering application / Biocompatibilidad de condrocitos humanos cultivados sobre un hidrogel a base de quitosano/alcohol polivnílico/epiclorhidrina para aplicación en ingeniería de tejidos

Tissue engineering (TE) has become an alternative for auricular reconstruction based on the combination of cells, molecular signals and biomaterials. Scaffolds are biomaterials that provide structural support for cell attachment and subsequent tissue development. Ideally, a scaffold should have characteristics such as biocompatibility and bioactivity to adequate support cell functions. Our purpose was to evaluate biocompatibility of microtic auricular chondrocytes seeded onto a chitosan-polyvinyl alcohol-epichlorohydrin (CS-PVA-ECH) hydrogel to propose this material as a scaffold for tissue engineering application. After being cultured onto CS-PVA-ECH hydrogels, auricular chondrocytes viability was up to 81%. SEM analysis showed cell attachment and extracellular matrix formation that was confirmed by IF detection of type II collagen and elastin, the main constituents of elastic cartilage. Expression of elastic cartilage molecular markers during in vitro expansion and during culture onto hydrogels allowed confirming auricular chondrocyte phenotype. In vivo assay of tissue formation revealed generation of neotissues with similar physical characteristics and protein composition to those found in elastic cartilage. According to our results, biocompatibility of the CS-PVA-ECH hydrogel makes it a suitable scaffold for tissue engineering application aimed to elastic cartilage regeneration.

La ingeniería de tejidos (TE) es una alternativa para la reconstrucción auricular basada en la combinación de células, señales moleculares y biomateriales. Los andamios fabricados con biomateriales brindan un soporte estructural que favorece la adhesión cellular y el desarrollo del tejido. Un andamio debe poseer características como biocompatibilidad y bioactividad para soportar adecuadamente funciones celulares. Nuestro objetivo fue evaluar la biocompatibilidad de condrocitos auriculares de microtia cultivados sobre un hidrogel a base de quitosano-alcohol polivinílico-epiclorhidrina (CS-PVA-ECH) y proponerlo como andamio con aplicaciones en ingeniería de tejidos. La viabilidad de los condrocitos auriculares es superior al 81% después de ser cultivados sobre el hidrogel. El análisis por SEM reveló la unión celular y formación de matriz extracellular sobre el hidrogel; confirmada mediante detección por IF de colágena tipo II y elastina. La expresión de marcadores moleculares durante la expansión in vitro y el cultivo sobre los hidrogeles confirmaron el fenotipo condral. El ensayo de formación de tejido in vivo demostró la generación de neotejidos con características físicas y composición similar al cartílago elástico. Nuestros resultados indican que la biocompatibilidad del hidrogel de CS-PVA-ECH lo hace un andamio adecuado para aplicaciones en ingeniería de tejidos enfocadas a regeneración de cartílago elástico.

A comparative evaluation of tray spacer thickness and repeat pour on the accuracy of monophasic polyvinyl siloxane impression material: In vitro study.

Purpose: This study was aimed to determine the effect of various tray spacer thickness and subsequent repeated pours on the accuracy and dimensional stability of the impression made from monophasic polyvinyl siloxane material. Materials and Methods: Custom trays with different spacer thickness (2, 4 and 6 mm) were used for making an impression of a master model simulating 3 unit fixed partial denture with monophasic polyvinyl siloxane material. These impressions were poured with die stone and repoured. Distance between the reference points were measured and subjected to statistical analysis. Result: Casts obtained from 1 st , 2 nd and 3 rd pour of the impression in 2, 4 and 6 mm spacer thickness tray have similar dimensional accuracy amongst each other and with the master model except in molar diameter and inter-abutment distances of cast obtained from 6 mm spacer thickness tray. Conclusion: The vertical distance of stone dies were decreased, whereas horizontal distance increased as the thickness of impression material is increased. There were statistically non-significant changes occurring among the repeated pours in 2, 4 and 6 mm spacer thickness. Clinical Implication: 2 and 4 mm spacer thickness are acceptable for making an impression for three unit fixed partial denture with monophasic polyvinyl siloxane material and it was not affected by two subsequent (1 st and 2 nd ) repeated pours.

Effect of five brands of latex gloves on the setting time of polyvinyl siloxane putty impression materials.

Addition silicone impression materials have been used as impression material for more than 20 years. Although they are among the most expensive impression materials, they became popular during the past decade as they have excellent physical properties. Prevention of infection is an important aspect in dental treatment since dental professionals are routinely exposed to the wide variety of microorganisms present in saliva. Gloves are the most common protective measure used during dental treatment. The gloves are mostly made of latex. In this study, we examine how the setting time of three types polyvinyl putty materials were affected by the use of five different brands of latex gloves and one brand of vinyl gloves. Each material was first mixed without wearing gloves according to the manufacturer's instructions. After the stipulated mixing time, the setting time was measured using the Vicat needle. The setting time is measured from the time of mixing till the time that the needle does not produce any indentation on the surface of the material. The putty material was then mixed with gloved hands (using the five different brands of latex gloves in turn) and the setting time was measured. Then the material was mixed with washed gloved hands, and the setting time was measured again. Finally, the material was mixed with vinyl gloved hands and the setting time was measured. The following conclusions were drawn from the study: Reprosil™ and Express™ showed significant variation in the setting time with the latex gloved hands. There was no significant variation in the setting time when material was mixed with unwashed vs washed gloved hands. Vinyl gloves did not significantly affect the setting time of any of the putty impression materials.

Effects of sulfur-based hemostatic agents and gingival retraction cords handled with latex gloves on the polymerization of polyvinyl siloxane impression materials

OBJECTIVES: This study investigated the possible interactions between three addition silicone materials (Express®, Aquasil Ultra® and Adsil®), three hemostatic agents (ferric sulfate, StatGel FS®; aluminum sulfate, GelCord®; and aluminum chloride, Hemostop®) and gingival retraction cords previously handled with latex gloves to determine whether direct contact with medicaments or indirect contamination by latex in conditions similar to those found in clinical practice inhibit or affect the setting of the impression materials. MATERIAL AND METHODS: A portable device for the simultaneous test of several specimens was specifically developed for this study. Polymerization inhibition was analyzed by examination of the impressions and the molded surface. Ten trials were performed for each addition silicone material used in the study, at a total of 240 study samples. RESULTS: All the samples tested (N=240) were nonreactive regardless of the type of combination used. CONCLUSIONS: Aluminum sulfate, ferric sulfate and aluminum chloride hemostatic solutions did not show any inhibitory potential on the addition silicone samples under study, and there were no changes in polymerization as a result of contact between addition silicone and retraction cords handled with latex gloves.

Dimensional accuracy and detail reproduction of two hydrophilic vinyl polysiloxane impression materials tested under different conditions.

Background and Objectives: A limitation of vinyl polysiloxane (VPS) impression materials is hydrophobicity, and manufacturers have added surfactants and labeled these new products as "hydrophilic." The purpose of this investigation was to evaluate and compare the dimensional accuracy and surface detail reproduction of two hydrophilic VPS impression materials under dry, moist, and wet conditions. Materials and Methods: Ten impressions were made under dry, moist, and wet conditions respectively, with monophase, and regular body VPS impression material using a stainless steel metal die similar to that described in American Dental Association (ADA) specification 19, with lines scribed on it. Dimensional accuracy was measured by comparing the average length of the middle horizontal line in each impression to the same line on the metal die, by using a measuring microscope. The surface detail was evaluated. A one-way analysis of variance and Student t-test were used to compare mean dimensional changes (α = 0.05). Results: Conditions (dry, moist, and wet) did not cause significant adverse effects on the dimensional accuracy of either material. The mean dimensional changes were 0.00084% (+0.00041%) for monophase and 0.00119% (+0.00033%) for regular body. Monophase material was satisfactory in detail reproduction 100% of the time in dry conditions, 90% in moist, and only 20% in wet conditions. The regular body showed 100% satisfactory impressions in dry, 80% in moist, and 10% in wet conditions. With the additional smooth surface evaluation, only under dry conditions impressions with clinically acceptable surface quality were produced. Conclusions: Dimensional changes for both materials were well within ADA standards of minimal shrinkage value of 0.5%.

Efficacy of five rotary systems versus manual instrumentation during endodontic retreatment

The purpose of this study was to evaluate the efficiency of ProFile, GT, ProTaper, Race and K3 rotary instruments compared with hand K-files for removal of gutta-percha during retreatment. Sixty mandibular premolars were instrumented with GT rotary files and filled by thermomechanical compaction of gutta-percha and AH Plus sealer. The teeth are randomly divided into 6 groups of 10 specimens each. The roots were split longitudinally, digital images were created using a flatbed scanner, and the areas with remaining filling material were demarcated using Image Tool 1.21 software. The results indicate that GT left significantly less (p<0.05) remaining filling material (1.18 ± 1.47) than hand (3.70 ± 3.16) and Hero instruments (2.99 ± 2.58). There was no statistically significant difference (p<0.05) among the others techniques: ProFile (1.99 ± 2.66), ProTaper (2.00 ± 1.99) and K3 (2.71 ± 2.87) when compared with GT. In conclusion, GT, ProFile, ProTaper and K3 were more effective in removing gutta-percha than manual and Hero instruments.

O propósito deste estudo foi avaliar a eficiência dos instrumentos rotatórios ProFile, GT, ProTaper, Race e K3 comparados com limas K para a remoção do material obturador durante o retratamento. Sessenta pré-molares inferiores foram instrumentados com o sistema rotatório GT e obturados usando a técnica de compactação termomecânica de guta-percha com cimento AH Plus. Os dentes foram divididos aleatoriamente em seis grupos de dez espécimes cada. As raízes foram seccionadas longitudinalmente, imagens digitais foram criadas através de um scanner, e as áreas de remanescentes de material foram medidas usando o programa Image Tool 1.21. Os resultados indicaram que o GT deixou significantemente menos material remanescente (1,18 ± 1,47) do que os instrumentos manuais (3,70 ± 3,16) e os instrumentos Hero (2,99 ± 2,58) (p<0,05). Não houve diferença estatisticamente significante entre as outras técnicas ProFile (1,99 ± 2,66), ProTaper (2,00 ± 1,99) e K3 (2,71 ± 2,87) quando comparadas com o GT. Como conclusão, GT, ProFile, ProTaper e K3 foram revelados como mais efetivos em remover a guta-percha do que os instrumentos manuais e os instrumentos Hero.

Development of meloxicam formulations utilizing ternary complexation for solubility enhancement

Meloxicam [an oxicam derivative], a relatively new cyclo-oxygenase inhibitor, is a member of enolic acid group of non-steroidal anti-inflammatory drugs. It is generally used in the treatment of rheumatoid arthritis, osteoarthritis and other joint pains. Meloxicam is practically insoluble in water [8 microg/ml], which directly influences the C[max], T[max], as well as the bioavailability of the drug. In the present study, an attempt has been made to improve the dissolution of Meloxicam by preparation of its solid dispersion using p-cyclodextrin blended with various water soluble polymer carriers i.e., HPMC [methocel IH], methylcellulose [400cps], PVP K30, HPMC [K[4]M], HPMC [50cps]. It is reported that when small amount of water soluble polymer is added to beta-cyclodextrin, its nature of solubilization significantly increases due to increase in the apparent complex stability constant. Phase solubility studies were carried out to evaluate the solubilizing power of beta-cyclodextrin along with various water soluble polymers. The solid dispersion was prepared and formulated into tablets and suspension, which were evaluated on the basis of various official tests. All the studies suggest that formulations of Meloxicam utilizing solid dispersion technique significantly enhances solubility [90 microg/ml] of the drug and results in superior formulations of the drug by using beta-cyclodextrin blended with 0.12% w/w HPMC [Methocel IH]. Ternary complexation is a valuable tool for solubility enhancement of drugs

A comparative study of inter-abutment distance of dies made from full arch dual-arch impression trays with those made from full arch stock trays: An in vitro study.

Objectives: The dual-arch impression technique is convenient in that it makes the required maxillary and mandibular impressions, as well as the inter-occlusal record in one procedure. The accuracy of inter-abutment distance in dies fabricated from dual-arch impression technique remains in question because there is little information available in the literature. Materials and Methods: This study was conducted to evaluate the accuracy of inter-abutment distance in dies obtained from full arch dual-arch trays with those obtained from full arch stock metal trays. Results and Conclusion: The metal dual-arch trays showed better accuracy followed by the plastic dual-arch and stock dentulous trays, respectively, though statistically insignificant. The pouring sequence did not have any effect on the inter-abutment distance statistically, though pouring the non-working side of the dual-arch impression first showed better accuracy.

Estudo de ação da saliva nas propriedades mecânicas de protetores bucais para esporte* / Study on the effect of saliva on the effect of saliva on the mechanical properties of mouth protectors for sports

Introdução - A eficiência e a durabilidade de protetores bucais para esporte dependem diretamente da forma com que são usados, pois sempre se apresentam em condições básicas de presença ou ausência de saliva. Para observar se essa condição de uso interfere em suas propriedades mecânicas este trabalho observa através de modelo experimental de arcos dentais obtidos em epóxi, acoplados a uma máquina universal de ensaios Kratos programada para movimento de compressão, o comportamento mecânico de protetores bucais para esporte, confeccionados em copolímero de etileno e acetato de vinila - EVA, posicionados no arco superior. Material e Métodos - Foram formados dois grupos de estudo: protetores bucais secos e protetores bucais saturados em saliva artificial, sendo estes últimos obtidos através de análise de saturação com o auxílio de balança analítica para observação de ganho de massa. As propriedades mecânicas observadas foram força máxima e energia absorvida. Resultados - Os resultados foram submetidos a análise estatística t-student (p < 0,05). Observou-se que no grupo dos protetores bucais saturados com saliva houve redução da força máxima (p = 0,00) devido a plastificação do material, responsável pela redução das forças intermoleculares ocasionando maior deformabilidade do protetor atestado pelo aumento da energia absorvida (p = 0,05) quando comparado ao grupo dos protetores secos. Conclusão - Conclui-se que a presença da saliva altera o comportamento mecânico do protetor bucal confeccionado em EVA tornando-o mais dúctil, portanto diminuindo a probabilidade de fraturas dentais e/ou lesões em tecidos circunvizinhos

Effect of conventional and experimental gingival retraction solutions on the tensile strength and inhibition of polymerization of four types of impression materials

In the present study, two types of tests (tensile strength test and polymerization inhibition test) were performed to evaluate the physical and chemical properties of four impression materials [a polysulfide (Permlastic), a polyether (Impregum), a condensation silicone (Xantopren) and a polyvinylsiloxane (Aquasil) ,3; when polymerized in contact with of one conventional (Hemostop) and two experimental (Vislin and Afrin) gingival retraction solutions. For the tensile strength test, the impression materials were mixed and packed into a steel plate with perforations that had residues of the gingival retraction solutions. After polymerization, the specimens were tested in tensile strength in a universal testing machine. For the polymerization inhibition test, specimens were obtained after taking impressions from a matrix with perforations that contained 1 drop of the gingival retraction solutions. Two independent examiners decided on whether or not impression material remnants remained unpolymerized, indicating interference of the chemical solutions. Based on the analysis of the results of both tests, the following conclusions were reached: 1. The tensile strength of the polysulfide decreased after contact with Hemostop and Afrin. 2. None of the chemical solutions inhibited the polymerization of the polysulfide; 3. The polyether presented lower tensile strength after polymerization in contact with the three gingival retraction agents; 4. The polyether had its polymerization inhibited only by Hemostop; 5. None of the chemical solutions affected the tensile strength of the condensation silicone; 6. Only Hemostop inhibited the polymerization of the condensation silicone; 7. The polyvinylsiloxane specimens polymerized in contact with Hemostop had significantly lower tensile strength; 8. Neither of the chemical solutions (Afrin and Vislin) affected the tensile strength of the polyvinylsiloxane and the condensation silicone; 9. Results of ...

2, 4 dichlorophenol (2, 4-DCP) sorption from aqueous solution using granular activated carbon and polymeric adsorbents and studies on effect of temperature on activated carbon adsorption.

Adsorption equilibrium, kinetics and thermodynamics of 2,4-dichlorophenol (2,4-DCP), one of the most commonly used chlorophenol, onto bituminous coal based Filtrasorb-400 grade granular activated carbon, were studied in aqueous solution in a batch system with respect to temperature. Uptake capacity of activated carbon found to increase with temperature. Langmuir isotherm models were applied to experimental equilibrium data of 2, 4-DCP adsorption and competitive studies with respect to XAD resin were carried out. Equilibrium data fitted very well to the Langmuir equilibrium model. Adsorbent monolayer capacity 'Q0, Langmuir constant 'b' and adsorption rate constant 'k(a)' were evaluated at different temperatures for activated carbon adsorption. This data was then used to calculate the energy of activation of adsorption and also the thermodynamic parameters, namely the free energy of adsorption, deltaG0, enthalpy of adsorption, deltaH0 and the entropy of adsorption deltaS0. The obtained results showed that the monolayer capacity increases with the increase in temperatures. The obtained values of thermodynamic parameters showed that adsorption of 2,4 DCP is an endothermic process. Synthetic resin was not found efficient to adsorb 2,4 DCP compared to activated carbon. The order of adsorption efficiencies of three resins used in the study found as XAD7HP > XAD4 > XAD1180.

Evaluación de la capacidad selladora de un cemento endodóntico experimental a base de polvo del Pro Root (MTA) con una resina de base acuosa como vehículo / Evaluation of the sealing ability of an experimental endodontic sealer based on Pro Root powder (MTA), with an aqueous-based resin as a vehicle

En el presente estudio se evaluó, mediante el método de filtración de fluidos, la capacidad de sellado coronario obtenido con un cemento endodóntico a base de polvo del Pro Root MTA con una resina polivinílica de base acuosa como vehículo. En 46 incisivos superiores y caninos superiores e inferiores con un conducto radicular, las coronas fueron seccionadas y los conductos radiculares instrumentados y divididos en 2 grupos de 20 piezas cada uno, dejando 6 dientes para los grupos control. En el grupo A, los conductos fueron obturados mediante la técnica de condensación lateral con conos de gutapercha y el cemento endodóntico experimental. En el grupo B, fueron obturados con la misma técnica utilizando AH-26 como sellador. Las mediciones fueron realizadas durante 10 minutos a 1,2 At de presión, a los 7 y 14 días de la obturación. A los 30 días, las mediciones se hicieron por un lapso de 60 minutos y se establecieron los valores promedios en volumen de cada grupo. Comparando los valores promedios de filtración de cada grupo a los 7, 14 y 30 días, el grupo experimental A presentó menor capacidad selladora a los 7 días, aunque ésta fue aumentando progresivamente, para alcanzar a los 30 días niveles de filtración menores al grupo control B, siendo la diferencia estadísticamente significativa (P<0.05). A la luz de los resultados concluimos que el sellado coronario con el cemento endodóntico experimental a base de polvo de ProRoot con una resina polivinílica de base acuosa, alcanzó niveles de filtración aceptables, comparativamente con la condensación lateral de conos de gutapercha cuando se utilizó sellador AH-26.

Mechanical behavior of Ethylene Vinyl Acetate copolymer (EVA) used for fabrication of mouthguards and interocclusal splints

In the present investigation, an experimental dental arch model fabricated in epoxy was assayed in Kratos universal testing machine to study the mechanical behavior of ethylene and vinyl acetate copolymer (EVA) in the form of mouthguard for sports and flat plate. The following variables were considered: thickness (3 and 4-mm plates), temperature (room and mouth temperature) and presence/absence of artificial saliva. Mechanical properties of EVA were tested under compressive strength: apparent absorbed energy (J.mm-1), maximum tension (N.mm-1), maximum dislocation (mm) and maximum strength (N). Data were recorded and modeled mathematically. Regarding the absorbed energy, maximum tension and maximum force, it was verified that the higher the thickness of the mouthguards, the better the results of force dissipation and redirection to the system and to several regions of the dental arch. In the presence of saliva and close to mouth temperature, the material responded positively to these alterations, resenting increased ductibility as well as improved mechanical responses. Regarding maximum dislocation, it was observed a better accommodation of the occlusion under conditions that simulate those observed in the oral environment. In conclusion, EVA proved to be an adequate material for fabrication of mouthguards and interocclusal splints. In addition, EVA showed good results in force dissipation and demonstrated a shock-absorbing capacity and a great protection potential.

Um modelo experimental de arco dentário, obtido em epóxi, acoplado a uma máquina universal de ensaios Kratos, foi utilizado para estudar o comportamento mecânico do copolímero de etileno e acetato de vinila (EVA), na forma de protetor bucal para esporte e placa plana. As seguintes variáveis foram observadas: espessura (lâminas de 3 e 4 mm), temperatura (ambiente e bucal) e presença ou ausência de saliva artificial. As propriedades mecânicas do EVA, foram testadas ao esforço compressivo: energia aparente absorvida (J.mm-1), tensão máxima (N.mm-1), deslocamento máximo (mm) e força máxima (N). Dados foram registrados e modelados matematicamente. Considerando a energia aparente absorvida, tensão máxima e força máxima, verificou-se que com o aumento da espessura dos protetores bucais houve melhores resultados de dissipação de forças compressivas e seu redirecionamento para o sistema e diversas regiões do arco dental. Com a presença de saliva e temperatura próxima a bucal o material respondeu positivamente a essas alterações aumentando sua ductibilidade apresentando assim melhora em sua resposta mecânica. Com respeito ao deslocamento máximo observou-se que houve melhor acomodação da oclusão quando em condições próximas à bucal. Conclue-se que o EVA provou ser um material adequado para a confecção de protetores bucais para esporte e placas interoclusais.O EVA mostrou melhores resultados na dissipação de forças demonstrando sua grande capacidade amortecedora e grande potencial de proteção.

Citotoxicidad de un sellador experimental a base de trióxido mineral / Citotoxicity of a mineral trioxide based experimental root canal sealer

El objetivo de este estudio fue determinar la citotoxicidad no específica de un sellador endodóntico experimental a base del polvo de ProRoot (MTA) mezclado con una resina polivinílica de base acuosa como vehículo en reemplazo del agua destilada, comparándolo con un material original, mediante la técnica de extendido en agar. Para el estudio se utilizaron 18 placas de Petri conteniendo las células L-929 en agar estéril, a las que se les adicionó solución de rojo neutro. Muestras preparadas de cemento Pro Root MTA y polvo de Pro Root MTA + resina polivinílica fueron colocadas en el interior de anillos circulares de silicona. Los controles positivos consistieron en los mismos anillos de silicona con hidróxido de Na al 10 por ciento y para los controles negativos se utilizaron los anillos vacíos. Todos los anillos fueron esterilizados con luz ultravioleta. Se colocaron 4 anillos por placa, conniendo los materiales a estudiar junto con los controles y se incubaron durante 24 hs en estufa gaseada a 37ºC en atmósfera de CO2, al 5 por ciento saturada de humedad. Las placas se examinaron utilizando un microscopio de óptica invertida con ocular micrométrico y se determinó el índice de decoloración y de lisis para cada especimen. Los resultados obtenidos indicaron que las muestras de Pro Root y polvo de Pro Root con una resina polivinílica presentaron el mismo nivel de citotoxicidad, ya que el área de decoloración y lisis se circunscribió a la superficie que se encontraba justo por debajo del material ensayado. En el caso del control positivo (hidróxido de sodio) el halo de decoloración y lisis superó los 6mm desde el material ensayado. El control negativo no presentó decoloración ni lisis, aún debajo de la superficie del mismo. A la luz de los resultados obtenidos con la metodología utilizada podemos concluir que el uso de una emulsión acuosa de alcoholes polivinílicos mezclado con polvo de Pro Root (MTA) no altera la citotoxicidad del material original.

Effect of microwave disinfection on denture base adaptation and resin surface roughness

This study evaluated the effect of disinfection methods [chemical disinfection (immersion in 100 ppm chloride solution) or microwave disinfection (690 W for 6 min)] on the internal adaptation of denture bases and resin surface roughness. For the adaptation test, 18 maxillary denture bases were obtained from stone casts duplicated from a metallic master model and submitted to the following treatments: 1) control (no disinfection), 2) chemical disinfection or 3) microwave disinfection. Disinfection procedures were performed twice (T1, T2) with a 7-day interval between them. Internal adaptation was measured at baseline (T0) and after T1 and T2 by weighing a vinyl polysiloxane film reproducing the gap between the resin base and the master model. For surface roughness measurement, 60 rectangular (5x10x40 mm) resin specimens were either mechanically or chemically polished and then submitted to the disinfection treatments. Surface roughness (Ra) was recorded after polishing (T0) and after T1 and T2. Data were analyzed by ANOVA GLM for repeated measures and Bonferroni correction at 5 percent significance level. Bases submitted to microwave disinfection had gradual increase of misfit, while bases immersed in chloride solution did not differ from the control group. Surface roughness increased in the mechanical polishing groups with microwave disinfection and decreased in the chemical polishing groups.

Este estudo avaliou o efeito de métodos de desinfecção [desinfecção química (imersão em solução clorada 100 ppm) ou desinfecção por microondas (690 W por 6 min)] na adaptação interna de bases de próteses totais e na rugosidade superficial da resina. Para o teste de adaptação, 18 bases superiores foram duplicadas a partir de um modelo-mestre metálico e submetidas aos seguintes tratamentos: 1) controle (sem desinfecção), 2) desinfecção química ou 3) desinfecção por microondas. Os procedimentos de desinfecção foram realizados duas vezes (T1, T2) com intervalo de 7 dias. A adaptação interna foi medida no baseline (T0), após T1 e T2 por pesagem de um filme de silicona de adição que reproduzia o espaço entre base de resina e modelo-mestre. Para a rugosidade superficial, 60 espécimes retangulares (5x10x40 mm) receberam polimento mecânico ou químico e foram submetidos aos tratamentos de desinfecção. A rugosidade superficial (Ra) foi registrada após o polimento (T0) e após T1 e T2. Os dados foram analisados por ANOVA GLM para medições repetidas e teste de Bonferroni com nível de significância de 5 por cento. As bases submetidas à desinfecção por microondas tiveram aumento gradual de desadaptação, enquanto que as bases imersas em solução clorada não diferiram do grupo controle. A rugosidade superficial aumentou nos grupos de polimento mecânico e desinfecção por microondas e diminuiu nos grupos de polimento químico.

Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane.

A simple procedure for the purification of tryptic peptides, prior to mass spectrometric analysis, using polyvinylidene fluoride membrane (PVDF) is described. The sensitivity of mass spectrometric analysis is such that minor impurities in tryptic peptide digests suppress the signal obtained. However, we obtained useful signal, from a sample that did not yield any spectra earlier, by purifying the sample using PVDF membrane. For this, the tryptic peptide digest was first spotted on the membrane which was then air-dried and washed. Further, the membrane was extracted with trifluoroacetic acid (TFA) and acetonitrile and subjected to mass spectrometric analysis. This procedure enabled us to identify a cross-reactive D1 antigen on the neutrophil surface that bound antibodies that targeted 60 kD Ro autoantigen in systemic lupus erythematosus, an autoimmune disorder.

A radioimmunoassay method for detection of DNA based on chemical immobilization of anti-DNA antibody

High selectivity provided by biomolecules such as antibodies and enzymes has been exploited during the last two decades for development of biosensors. Of particular importance are efficient immobilization methods for biomolecules in order to preserve their biological activities. In this study, we have evaluated immobilization strategies for an anti-DNA antibody on a self-assembled monolayer of omega-functionalized thiols. The antibody was immobilized via peptide bond formation between the primary amines in the antibody and the carboxyl groups on the self-assembled monolayer. The peptide bond coupling was achieved by activating COOH groups on the surface through N-Hydroxysuccimide (NHS)-ester formation, followed by acylation of NH2 group in the antibody. DNA binding activity of the immobilized antibody was examined by counting beta emission from 35S-labeled DNA.